NMR 400 MHz
Structure confirmation and assay without a reference standard.
Nuclear magnetic resonance is the only technique that unambiguously confirms a compound's structure. Our qNMR (quantitative NMR) determines content without requiring a purity reference standard — just an internal standard.
What we measure
- Structure confirmation (1D: ¹H, ¹³C, DEPT; 2D: COSY, HSQC, HMBC, NOESY)
- qNMR — content determination without a purity standard
- Unknown impurity identification (structural elucidation)
- Residual solvent analysis (¹H NMR, complementary to GC-MS)
- Conformational isomer / tautomer ratios
- Stability studies in matrix (degradation monitoring)
- Polymorph and crystal form analysis (ssNMR, on request)
50–100 mg soluble substance, preferred solvents: DMSO-d6, CDCl3, CD3OD. For qNMR minimum 20 mg. Ship in sealed container — solvent chosen on-site.
Instruments + methods
Technical specs- System
- Bruker Avance III HD 400 MHz
- Probe
- 5 mm BBFO with z-gradient
- Automation
- SampleXpress 24-position
- Temp. control
- −150 °C to +150 °C (±0.1 °C)
- Spectral database
- SDBS + internal (50 000+ spectra)
- Accreditation
- ISO/IEC 17025 (scope AL-1142/NMR)
from 34 900 ISK
5–10 days · full 2D characterization up to 3 weeks
Related questions
02 Q- 01
How does qNMR differ from HPLC-UV for content determination?
qNMR is 'absolute' — no purity standard for the target compound is required. For novel molecules without available standards it's the only way to obtain a true number. HPLC requires a known concentration reference.
- 02
Do you have a cryoprobe?
No — we use a BBFO probe which covers 95 % of jobs. For extremely low-concentration samples (below 1 mg) we collaborate with University of Iceland (cryoprobe 600 MHz).